irf3 antibody Search Results


rabbit anti irf3  (MedChemExpress)
94
MedChemExpress rabbit anti irf3
Rabbit Anti Irf3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody/pmc12935302-83-45-50
Average 94 stars, based on 1 article reviews
rabbit anti irf3 - by Bioz Stars, 2026-09
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mouse monoclonal igg1 anti irf3  (Novus Biologicals)
90
Novus Biologicals mouse monoclonal igg1 anti irf3
Mouse Monoclonal Igg1 Anti Irf3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody+(3F10)+-+BSA+Free/bio_rxiv__2020__03__19__994988-198-176-181
Average 90 stars, based on 1 article reviews
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irf3  (Proteintech)
96
Proteintech irf3
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Irf3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody/pmc12872204-85-39-41
Average 96 stars, based on 1 article reviews
irf3 - by Bioz Stars, 2026-09
96/100 stars
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anti sting  (Proteintech)
96
Proteintech anti sting
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Anti Sting, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/TMEM173%2FSTING+Antibody/pmc13024547-72-28-30
Average 96 stars, based on 1 article reviews
anti sting - by Bioz Stars, 2026-09
96/100 stars
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irf  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology irf
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Irf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF-3+Antibody/10__1074_slash_jbc__m400938200-67-8-4
Average 96 stars, based on 1 article reviews
irf - by Bioz Stars, 2026-09
96/100 stars
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anti irf3  (Aviva Systems)
90
Aviva Systems anti irf3
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Anti Irf3, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody+(OALA02175)/pmc03849481-131-24-27
Average 90 stars, based on 1 article reviews
anti irf3 - by Bioz Stars, 2026-09
90/100 stars
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anti phospho s33 rpa32  (Bethyl)
93
Bethyl anti phospho s33 rpa32
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Anti Phospho S33 Rpa32, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody/pm37697435-201-29-31
Average 93 stars, based on 1 article reviews
anti phospho s33 rpa32 - by Bioz Stars, 2026-09
93/100 stars
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mouse antibody against sting  (R&D Systems)
93
R&D Systems mouse antibody against sting
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Mouse Antibody Against Sting, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/Mouse+IRF3+Antibody/pmc09417302-20-0-7
Average 93 stars, based on 1 article reviews
mouse antibody against sting - by Bioz Stars, 2026-09
93/100 stars
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anti irf3  (Novus Biologicals)
94
Novus Biologicals anti irf3
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Anti Irf3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Antibody/pmc08531670-214-38-39
Average 94 stars, based on 1 article reviews
anti irf3 - by Bioz Stars, 2026-09
94/100 stars
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anti phospho irf3  (OriGene)
90
OriGene anti phospho irf3
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Anti Phospho Irf3, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/IRF3+Rabbit+Polyclonal+Antibody/pmc05397474-61-39-40
Average 90 stars, based on 1 article reviews
anti phospho irf3 - by Bioz Stars, 2026-09
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p irf3 s386  (Boster Bio)
94
Boster Bio p irf3 s386
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
P Irf3 S386, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/Anti-IRF3+Antibody/pmc11742825-4-0-3
Average 94 stars, based on 1 article reviews
p irf3 s386 - by Bioz Stars, 2026-09
94/100 stars
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rabbit  (Boster Bio)
90
Boster Bio rabbit
USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, <t>IRF3,</t> and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.
Rabbit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf3+antibody/Anti-TMEM173+Antibody+Picoband/pmc12897489-9-4-6
Average 90 stars, based on 1 article reviews
rabbit - by Bioz Stars, 2026-09
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Image Search Results


USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, IRF3, and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.

Journal: The FASEB Journal

Article Title: Cardiomyocyte‐Derived USP20 Attenuates Diabetic Cardiomyopathy by Facilitating the Degradation of STING and Mitigating STING‐Mediated Inflammation

doi: 10.1096/fj.202503913R

Figure Lengend Snippet: USP20 attenuates inflammatory response via modulation of the STING signaling pathway. (A, B) Expression of STING, TBK1, p‐TBK1, IRF3, and p‐IRF3 in cardiomyocytes overexpressing USP20 induced by HG + PA tested by western blotting (A) and the statistical results, data has been normalized against GAPDH (B). n = 5. (C) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in cardiomyocytes overexpressing USP20 induced by HG + PA. n = 5. (D, E) Expression of STING, TBK1, p‐TBK1, IRF3 and p‐IRF3 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA tested by western blotting (D) and the statistical results, data have been normalized against GAPDH (E). n = 5. (F) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 and Vcam1 in cardiomyocytes transfected with si‐USP20 or si‐NC and induced by HG + PA. n = 5. (G) The mRNA expression levels of Il‐1β, Il‐6, Il‐18, Tnf‐α, Cxcl1, Ccl2, Icam1 , and Vcam1 in heart tissues of USP20 fl/fl non‐diabetic controls, USP20 conditional knockout (CKO) non‐diabetic controls, USP20 fl/fl diabetic mice, and USP20 CKO diabetic mice. Data are expressed as the mean ± SEM. *** p < 0.001; ** p < 0.01; * p < 0.05, p > 0.05, ns: No differences. n : The number of independent biological replicates.

Article Snippet: Primary antibodies against GAPDH (1:1000, HUABIO, ET1601‐4), USP20 (1:1000, proteintech, 17491‐1‐AP), MyHC (1:1000, HUABIO, ET1702‐88), ANP (1:200, SANTA, sc‐515701), STING (1:1000, HUABIO, HA722832), P62 (1:1000, HUABIO, HA721171), p‐STING (1:1000, HUABIO, HA723137), TBK1 (1:1000, proteintech, 28397‐1‐AP), p‐TBK1 (1:1000, proteintech, 82383‐1‐RR), IRF3 (1:1000, proteintech, 11312‐1‐AP), p‐IRF3 (1:1000, proteintech, ET1705‐81), HA (1:1000, proteintech, 51064‐2‐AP).

Techniques: Expressing, Western Blot, Transfection, Knock-Out